Laser Speckle Contrast Imaging System vs Laser Doppler Flowmetry
Laser Doppler Flowmetry (LDF) has been used for decades as a golden standard for confirming a successful stroke model by showing at least 75% blood flow decrease from baseline. LDF detects blood flow changes in a focal spot limited by its probe size, and presents the data as a linear graph with no sptial resolution. Although LDF can provide perfusion imaging by scanning LDF (SLDF) or laser doppler imaging (LDI), but its spatial/temporal resolution could not meet the standards for a realtime monitring method. The LSCI is superior to SLDF/LDI by offering a full-field high-resolution fast imaging method which is about 100 times higher in sptial resolution, and almost 10 thousand times faster than SLDF/LDI. Obviously the emerging LSCI will be a much better method than LDF for in vivo confirmation of a successful stroke model and will become the upgraded golden standard from LDF in stroke modeling. Because of being able to provide quantitative analyses of perfusion data, the LSCI system could also help with the quality control of stroke models.

RFLSI-ZW laser speckle imaging system is based on laser speckle contrast imaging technology (LSCI). With its advanced optical design and improved image processing algorithm, RFLSI-ZW shows greater performance in image field size, image quality, full-field frame rate and optical resolution, and provides a powerful and efficient means for human and animal tissue microcirculation measurement. The system provides quantitative perfusion data and comprehensive analyses.

Typical images obtained in distal MCAO model (Wan et al 2022)

Highlights Of RFLSI-ZW

• It can image any exposed tissue (skin or surgically exposed tissues) and species.
• It provides quantitative perfusion data and comprehensive analyses.
• It is non-contact, and does not use contrast agents for visualing blood vessels.
• The built-in CMOS global shutter camera can achieve faster data acquisition and processing speed.
• Best optical resolution of 3.9 μm/pixel, providing more detailed tissue structures.
• Max frame rate (full field) up to 100 fps, acquiring real-time changes in larger areas.
• Motorized 10x optical zoom and auto focus.
• Image size ranges from 0.57×0.75 to 22.5×30 cm2 in all-in-one imager, covering multiple research applications.
• Fast auto focusin and fine manual focus.
• Optimal lens assembly which filters ambient and reflecting light.
• Class 1 of measuring and indicating lasers, safe to use without eye protection System
• Equiped with laser stability hardware enabling reliable and consistent measurement over minutes, hours and days.
• Calibration with calibration box. Self-calibration is possible at any time to keep the equipment in optimal working condition.
• Trigger In/Out BNC connections for communication with external devices.
• Unlimited installation of analysis software in PC.

• Maximum Camera Resolution: 2064×1544 pixels, offering clear and detailed imaging capabilities.
• Optimal Resolution: Achieves the best clarity at 3.9 μm/pixel, ensuring precise image capture.
• Image Capabilities: Supports multiple imaging modes including Flux, Gray, Intensity, Color, and Overlay for versatile imaging needs.
• Measurement Laser: Utilizes a 785 nm, Class 1 laser for accurate measurements.
• Indicating Laser: Features two 650 nm, Class 1 lasers for precise targeting and indication.
• Focus: Offers both Auto and Manual focusing options, with fine focus adjustment for optimal clarity.
• Trigger Inputs: Equipped with 2×BNC trigger inputs for external control.
• Image Size Range: Flexible imaging area from 0.57× 0.75 cm² up to 22.5×30 cm², accommodating a wide range of sample sizes.
• Maximum Frame Rate: Capable of capturing images at 100 FPS (full field), allowing for smooth and rapid imaging sequences.
• Zoom: Features a 10× zoom capability for detailed examination of specimens.
• Working Distance: Adjustable from 10 to 40 cm, providing continuous focus across a wide range.
• System Calibration: Includes a Calibration Box for ensuring measurement accuracy.
• Measurement Algorithms: Employs temporal and spatial processing for enhanced analysis.
• PC Connections: Comes with 1 x USB 3.0 port for fast and reliable data transfer.
• Software: Includes Acquisition Software and Analysis Software for comprehensive data capture and analysis.
• Power Supply: Supports Universal Voltage (100V-230V), with performance unaffected by the local electrical supply frequency.
• Stand Options: The scan head is compatible with a standard VESA mount, offering versatility with desktop stand, Microstand, and Clinical Mobile stand options.

Download the brochure for RFLSI ZW Laser Speckle Imaging System

Since 2019, our imaging system has been adopted by more than 100 colleges, universities, and research institutes worldwide such as Stanford University School of Medicine, university of manchester, UC Davis, Duke university. What’s more, it has contributed to publishing more than 60 reputed research papers in magazines like Nature communications, Blood, Diabetes, and Theranostic.

Selected References:

Wan, T., Zhu, W., Zhao, Y. et al. Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice. Nat Commun 13, 1134 (2022). https://doi.org/10.1038/s41467-022-28777-9

Yin, L., Yu, T., Cheng, L. et al. Laser speckle contrast imaging for blood flow monitoring in predicting outcomes after cerebral ischemia-reperfusion injury in mice. BMC Neurosci 23, 80 (2022). https://doi.org/10.1186/s12868-022-00769-x

Zhou, C, Zhu, T, Ni, W, et al. Gain-of-function of progesterone receptor membrane component 2 ameliorates ischemic brain injury. CNS Neurosci Ther. 2023; 00: 1- 17. https://doi.org/10.1111/cns.14122